Detection of Human and Mouse BMAL1 by Western Blot (h & m) and Immunoprecipitation (h).  Samples:  Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (T; 50 ug) cells.  Antibodies: Affinity purified rabbit anti-BMAL1 antibody A302-616A used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.  BMAL1 was also immunoprecipitated by rabbit anti-BMAL1 antibody BL9459, which recognizes an upstream epitope.  Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).