Detection of Human CKI delta by Western Blot and Immunoprecipitation.  Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CKI delta antibody A302-136A used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate.  CKI delta was also immunoprecipitated by a rabbit antibody (BL7891) directed to an epitope that is common to CKI epsilon and CKI delta.  CKI epsilon was immunoprecipitated and blotted using A302-135A. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).