Detection of c-myc-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein.     Antibodies: Affinity-purified, HRP-conjugated, goat anti-c-myc antibody A190-104P used for WB at 0.04 ug/ml (1:25,000).   Detection:  Chemiluminescence with an exposure time of 10 seconds.