Detection of c-myc-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein.     Antibodies: Affinity purified, goat anti-c-myc antibody A190-104A used for WB at 0.04 ug/ml (1:25,000).   Detection: Chemiluminescence with an exposure time of 30 seconds.