Fluorescent Western Blot Analysis Demonstrating Diminished Cross-reactivity of Donkey anti-Rabbit IgG Cross-adsorbed Secondary Antibody with Goat and Mouse IgG.  (A) HeLa whole cell lysate (30 ug) was blotted and incubated with a cocktail of mouse anti-hnRNP A500-011A (A500-011A-1), rabbit anti-actin A300-491A (A300-491A-5), and goat anti-GAPDH A303-878A (A303-878A-1) at 1 ug/ml each.  For detection the blot was incubated with 0.5 ug/ml donkey anti-rabbit IgG heavy and light chain cross-adsorbed antibody conjugated to Dylight® 680 A120-208D6 (A120-208D6-1). Donkey anti-rabbit IgG heavy and light chain cross-adsorbed antibody (A120-208D6) specifically detected rabbit anti-actin A300-491A (green) and showed no cross-reactivity with the goat anti-GAPDH and mouse anti-hnRNP primary antibodies.  (B) GAPDH, actin, and hnRNP  were detected In a parallel strip incubated with the primary antibody cocktail used in (A), and a secondary antibody cocktail of Dylight® 488-conjugated donkey anti-mouse A90-337D2 (A90-337D2-5) (blue), Dylight® 680-conjugated donkey anti-rabbit A120-208D6 (A120-208D6-1) (green), and Dylight® 800-conjugated donkey anti-goat A50-201D8 (A50-201D8-1) (red) at 0.5 ug/ml each.