Western blot analysis of brain lysate using SOX2 antibody. The lane on the left is treated with the antigen-specific peptide.

DF6109 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22!aC. An HRP conjugated goat anti-rabbit antibody was used as the secondary