Western blot analysis of CD19 (Phospho-Tyr500) using HeLa whole cell lysates

AF8467 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25!aC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37!aC. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod