Detection of Human AKAP8/AKAP95 by Western Blot and Immunoprecipitation.  Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-AKAP8/AKAP95 antibody A301-062A used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  AKAP8/AKAP95 was also immunoprecipitated by rabbit anti-AKAP8/AKAP95 antibody A301-061A, which recognizes an upstream epitope.  For blotting immunoprecipitated AKAP8/AKAP95, ReliaBLOTTM  Reagents and Procedures (Cat. No. WB120) were used.  Detection: Chemiluminescence with exposure times of 3 seconds (A and B).