抗Ki-67/MKI67抗体 | Anti-Ki-67/MKI67 Antibody

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Image

	Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [NB500-170] - IHC analysis of a formalin fixed paraffin embedded tissue section of human tonsil using 1:50 dilution of rabbit anti-KI67 antibody. The staining was developed with HRP labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Ki67 antibody generated a specific nuclear staining in the cells in germinal centers of the tested tonsil tissue.
	Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [NB500-170] - IHC analysis of a formalin fixed paraffin embedded tissue section of human tonsil using 1:200 dilution of rabbit anti-KI67 antibody. The staining was developed with HRP labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Ki67 antibody generated a specific nuclear staining in the cells in germinal centers of the tested tonsil tissue.
	Immunocytochemistry/Immunofluorescence: Ki-67/MKI67 Antibody [NB500-170] - Ki67 Antibody [NB500-170] - Confocal immunofluorescent analysis of MCF7 cells using Ki67 antibody (NB500-170, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
	Immunocytochemistry/Immunofluorescence: Ki-67/MKI67 Antibody [NB500-170] - Ki67 Antibody [NB500-170] - Ki67 antibody (1:100) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.
	Immunocytochemistry/Immunofluorescence: Ki-67/MKI67 Antibody [NB500-170] - Ki67 Antibody [NB500-170] - Ki67 antibody was tested at 1:25 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
	Western Blot: Ki-67/MKI67 Antibody [NB500-170] - Ki67 Antibody [NB500-170] - Western blot analysis of A431 (A) and Hek293 (B) cell lysate using Ki67 antibody (NB500-170) at 2 ug/ml.
	Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [NB500-170] - Ki67 Antibody [NB500-170] - Human tonsil.
	Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [NB500-170] - Ki67 staining within mouse aortic sinus. This image was submitted via customer review.

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Background

Originally discovered employing mouse monoclonal antibody against a nuclear antigen from Hodgkin's lymphoma-derived cell line, this non-histone protein was named Ki67 after researcher's location (Gerdes and colleagues), Ki for Kiel University in Germany and 67 referring to the clone number on the 96-well plate. It interacts with KIF15 as well as MKI67IP, and is suggested to be involved in cell cycle regulation. Ki67 is a large protein with expected molecular weight of about 395 kD and has a very complex localization pattern within the nucleus, one which changes during cell cycle progression. Its expression occurs specially during late G1, S, G2 and M phases of the cell cycle, while in cells undergoing G0 phase, Ki67 remains undetectable. Ki67 undergoes phosphorylation/dephosphorylation during mitosis, is susceptible to proteases and its structure implies that its expression is regulated by proteolytic pathways. Ki67 is associated with nucleolar DFC (dense fibrillary component) and its regulation appears to be tightly controlled (estimated half life is 60-90 min, regardless of the cell position in the cell cycle), presumably by precise synthesis and degradation systems involving proteasome, a protease complex. Due to its association with cell divison process, Ki-67 is routinely used as cellular proliferation marker of solid tumors as well as certain hematological malignancies, and a correlation has been demonstrated between Ki-67 index and the histopathological grade of cancers.

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