抗beta Actin抗体 | Anti-beta Actin antibody
掲載日情報:2020/04/13 現在Webページ番号:39650
GeneTex社の抗beta Actin抗体 | Anti-beta Actin antibodyです。
※ 本製品は研究用です。研究用以外には使用できません。
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特長
- 高品質の抗体です。
- 幅広い研究分野に関連する抗体を取り揃えています。
- 使用されたアプリケーションや動物種などの情報が充実しています。
- 多数の論文で使用実績がある信頼性の高い抗体です。
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価格
[在庫・価格 :2024年04月18日 20時55分現在]
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Anti-β-Actin, Rabbit-Poly |
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Anti-β-Actin, Rabbit-Poly |
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40 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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[在庫・価格 :2024年04月18日 20時55分現在]
Anti-β-Actin, Rabbit-Poly
文献数: 40
- 商品コード:GTX110564
- メーカー:GNT
- 包装:25μl
- 価格:¥24,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
レビューあり。KO/KDバリデーション済み抗体。 別名:actin beta,BRWS1,PS1TP5BP1 Genbank No: 60 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Drosophila/Human/Mouse/Rat/Yeast | 適用 | IC,IF,IHC,IP,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 |
ローディング・コントロール用抗体 使いっきり抗体 |
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関連記事 |
GeneTex社における抗体の品質管理 |
Anti-β-Actin, Rabbit-Poly
文献数: 40
- 商品コード:GTX110564
- メーカー:GNT
- 包装:100μl
- 価格:¥75,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
レビューあり。KO/KDバリデーション済み抗体。 別名:actin beta,BRWS1,PS1TP5BP1 Genbank No: 60 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Drosophila/Human/Mouse/Rat/Yeast | 適用 | IC,IF,IHC,IP,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 |
ローディング・コントロール用抗体 |
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関連記事 |
GeneTex社における抗体の品質管理 |
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DATA IMAGES
beta Actin antibody detects beta Actin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at a dilution of 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. | |
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with beta Actin antibody (GTX110564) diluted at 1:10000 and competitor's antibody (sc-130656) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product. | |
beta Actin antibody detects ACTB protein by western blot analysis. A. 30 μg drosophila lysate/extract 10% SDS-PAGE beta Actin antibody (GTX110564) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. | |
beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. beta Actin antibody (GTX110564) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min | |
beta Actin antibody detects beta Actin protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract 10% SDS-PAGE beta Actin antibody (GTX110564) dilution: 1:20000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. | |
beta Actin antibody detects beta Actin protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted by 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. | |
Immunoprecipitation of beta Actin protein from 293T whole cell extracts using 5 μg of beta Actin antibody (GTX110564). Western blot analysis was performed using beta Actin antibody (GTX110564). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent. | |
beta Actin antibody detects beta Actin protein at cytoplasm in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. beta Actin antibody (GTX110564) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min | |
Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. | |
beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. beta Actin antibody (GTX110564) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min | |
The WB analysis of beta Actin antibody was published by Ishii T and colleagues in the journal Aging Cell in 2017 . PMID: 27623715 | |
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. | |
Various whole cell extracts (15 | 5 | 2 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. | |
beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min. Green: beta Actin protein stained by beta Actin antibody (GTX110564) diluted at 1:500. Blue: Hoechst 33342 staining. | |
The WB analysis of beta Actin antibody was published by Ishii T and colleagues in the journal Aging Cell in 2017 . PMID: 27623715 | |
The WB analysis of beta Actin antibody was published by Ishii T and colleagues in the journal Aging Cell in 2017 . PMID: 27623715 | |
The WB analysis of beta Actin antibody was published by Ishii T and colleagues in the journal Redox Biol in 2014.PMID: 24936442 |
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製品情報
Host | Rabbit |
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Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P, IP, IHC |
Reactivity | Human, Mouse, Rat, Drosophila |
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APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.Application | Dilution |
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WB | 1:5000-1:20000 |
ICC/IF | 1:100-1:1000 |
IHC-P | 1:100-1:1000 |
IP | 1:100-1:1000 |
IHC | Assay dependent |
Calculated MW | 42 kDa. ( Note ) |
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Positive Control | 293T , A431 , HeLa , HepG2 , Neuro2A , CD830 , NIH-3T3 , Raw264.7 , C2C12 , mouse liver , rat liver , PC-12 , Rat2 , drosophila |
Predict Reactivity | Rabbit, Sheep, Bovine, Dog, Chicken, Guinea pig, Japanese Medaka, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity) |
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PROPERTIES
Form | Liquid |
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Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Concentration | 0.09 mg/ml (Please refer to the vial label for the specific concentration.) |
Antigen Species | Human |
Immunogen | Recombinant protein encompassing a sequence within the center region of human beta Actin. The exact sequence is proprietary. |
Purification | Purified by antigen-affinity chromatography. |
Conjugation | Unconjugated |
Note | For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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TARGET
Synonyms | actin beta , BRWS1 , PS1TP5BP1 |
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Cellular Localization | Cytoplasm , cytoskeleton |
Background | This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq] |
Database | ・ Gene ID: 60 ACTB ・ UniProt: P60709 ACTB |
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REFERENCE
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