抗beta Actin抗体 | Anti-beta Actin antibody

掲載日情報:2020/04/13 現在Webページ番号:39650

GeneTex社の抗beta Actin抗体 | Anti-beta Actin antibodyです。

本製品は研究用です。研究用以外には使用できません。

特長

  • 高品質の抗体です。
  • 幅広い研究分野に関連する抗体を取り揃えています。
  • 使用されたアプリケーションや動物種などの情報が充実しています。
  • 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

[在庫・価格 :2024年04月18日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-β-Actin, Rabbit-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 40
説明文
レビューあり。KO/KDバリデーション済み抗体。
別名:actin beta,BRWS1,PS1TP5BP1
Genbank No: 60
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Drosophila/Human/Mouse/Rat/Yeast 適用 IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 ローディング・コントロール用抗体
使いっきり抗体
関連記事 GeneTex社における抗体の品質管理
Anti-β-Actin, Rabbit-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 40
説明文
レビューあり。KO/KDバリデーション済み抗体。
別名:actin beta,BRWS1,PS1TP5BP1
Genbank No: 60
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Drosophila/Human/Mouse/Rat/Yeast 適用 IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 ローディング・コントロール用抗体
関連記事 GeneTex社における抗体の品質管理

[在庫・価格 :2024年04月18日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-β-Actin, Rabbit-Poly

文献数: 40

説明文 レビューあり。KO/KDバリデーション済み抗体。
別名:actin beta,BRWS1,PS1TP5BP1
Genbank No: 60
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Drosophila/Human/Mouse/Rat/Yeast 適用 IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 ローディング・コントロール用抗体
使いっきり抗体
関連記事 GeneTex社における抗体の品質管理

Anti-β-Actin, Rabbit-Poly

文献数: 40

説明文 レビューあり。KO/KDバリデーション済み抗体。
別名:actin beta,BRWS1,PS1TP5BP1
Genbank No: 60
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Drosophila/Human/Mouse/Rat/Yeast 適用 IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 ローディング・コントロール用抗体
関連記事 GeneTex社における抗体の品質管理



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DATA IMAGES

beta Actin antibody detects beta Actin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at a dilution of 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with beta Actin antibody (GTX110564) diluted at 1:10000 and competitor's antibody (sc-130656) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
beta Actin antibody detects ACTB protein by western blot analysis.
A. 30 μg drosophila lysate/extract
10% SDS-PAGE
beta Actin antibody (GTX110564) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
beta Actin antibody (GTX110564) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
beta Actin antibody detects beta Actin protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
10% SDS-PAGE
beta Actin antibody (GTX110564) dilution: 1:20000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
beta Actin antibody detects beta Actin protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted by 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunoprecipitation of beta Actin protein from 293T whole cell extracts using 5 μg of beta Actin antibody (GTX110564).
Western blot analysis was performed using beta Actin antibody (GTX110564).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
beta Actin antibody detects beta Actin protein at cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
beta Actin antibody (GTX110564) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
beta Actin antibody (GTX110564) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
The WB analysis of beta Actin antibody was published by Ishii T and colleagues in the journal Aging Cell in 2017 . PMID: 27623715
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (15 | 5 | 2 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min.
Green: beta Actin protein stained by beta Actin antibody (GTX110564) diluted at 1:500.
Blue: Hoechst 33342 staining.
The WB analysis of beta Actin antibody was published by Ishii T and colleagues in the journal Aging Cell in 2017 . PMID: 27623715
The WB analysis of beta Actin antibody was published by Ishii T and colleagues in the journal Aging Cell in 2017 . PMID: 27623715
The WB analysis of beta Actin antibody was published by Ishii T and colleagues in the journal Redox Biol in 2014.PMID: 24936442


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製品情報

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ApplicationWB, ICC/IF, IHC-P, IP, IHC
ReactivityHuman, Mouse, Rat, Drosophila


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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW 42 kDa. ( Note )
Positive Control 293T , A431 , HeLa , HepG2 , Neuro2A , CD830 , NIH-3T3 , Raw264.7 , C2C12 , mouse liver , rat liver , PC-12 , Rat2 , drosophila
Predict Reactivity Rabbit, Sheep, Bovine, Dog, Chicken, Guinea pig, Japanese Medaka, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)


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PROPERTIES

Form Liquid
Buffer 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration 0.09 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species Human
Immunogen Recombinant protein encompassing a sequence within the center region of human beta Actin. The exact sequence is proprietary.
Purification Purified by antigen-affinity chromatography.
Conjugation Unconjugated
Note For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


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TARGET

Synonyms actin beta , BRWS1 , PS1TP5BP1
Cellular Localization Cytoplasm , cytoskeleton
Background This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]
Database ・ Gene ID: 60 ACTB
・ UniProt: P60709 ACTB


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REFERENCE

Application ReferenceApplication/Reactivity
Khanal S et al. PLoS Pathog 2019; 15 (2) : e1007442 High-risk human papillomavirus oncogenes disrupt the Fanconi anemia DNA repair pathway by impairing localization and de-ubiquitination of FancD2.
Ryouichi Satou et al. Heliyon 2019; (Epub) Light conditions affect rhythmic expression of aquaporin 5 and anoctamin 1 in rat submandibular glands.Application : WB
Reactivity : Rat
Yi-Chang Wang et al. Nature Communication 2018; 9 : 3996 USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and β-TrCP and promotes cancer malignancy.Application : WB
Reactivity : Human
Nashine S et al. Sci Rep 2018; 8 (1) : 15175 Characterizing the protective effects of SHLP2, a mitochondrial-derived peptide, in macular degeneration.Application : WB
Reactivity : Human
Gong W et al. J Cell Biochem 2018; (Epub) Deletion of long noncoding RNA EFNA3 aggravates hypoxia-induced injury in PC-12 cells by upregulation of miR-101a.Application : WB
Reactivity : Rat


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お問い合わせ先

(テクニカルサポート 試薬担当)

reagent@funakoshi.co.jp

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