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Ubiquitin Activating Enzyme 組換え体(リコンビナント)タンパク質 | Recombinant Human Ubiquitin Activating Enzyme

掲載日情報：2018/02/08 現在Webページ番号：216419

アールアンドディー システムス /
R&D Systems, Inc.
[メーカー略称：RSD]

R&D Systems社製の高品質なUbiquitin Activating Enzymeの組換え体タンパク質(Recombinant Human Ubiquitin Activating Enzyme)です。
※本製品は研究用です。研究用以外には使用できません。

[在庫・価格 ：2024年04月19日 13時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF
 10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
説明文
M.W.：118 kDa，純度：>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.，ブランド：Boston Biochem，由来動物：Human，Genbank：7317，産生：Sf 21 (baculovirus)
別名：A1S9T
Genbank No： 7317
Protein Accession No： P22314
法規制等 カルタヘナ該当品
保存条件 -80℃ 法規備考
掲載カタログ

製品記事 ユビキチンのE1酵素（R&D Systems社）
ユビキチン・プロテアソーム特集
関連記事

[在庫・価格 ：2024年04月19日 13時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF

文献数： 0

説明文 M.W.：118 kDa，純度：>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.，ブランド：Boston Biochem，由来動物：Human，Genbank：7317，産生：Sf 21 (baculovirus)
別名：A1S9T
Genbank No： 7317
Protein Accession No： P22314
法規制等 カルタヘナ該当品
保存条件 -80℃ 法規備考
掲載カタログ

製品記事 ユビキチンのE1酵素（R&D Systems社）
ユビキチン・プロテアソーム特集
関連記事

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CF(Carrier-Free)とは？

R&D Systems社組換え体タンパク質製品では通常ウシ血清アルブミン(BSA)をキャリアタンパク質として加えています。キャリアタンパク質を加えることで組換え体タンパク質の安定性が高まり、使用期限が長くなります。また、より濃度の低い溶液での保管も可能となります。CF製品はBSAが含まれない製品となります。一般的に細胞培養や、ELISAのスタンダードにはBSA含有製品を推奨しています。CF製品はBSAが影響してしまうアプリケーションに推奨されます。

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Product Details

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

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Related Product & Information

Background
Ubiquitin-activating Enzyme/UBE1
Ubiquitin-activating Enzyme (UBE1), also known as Ubiquitin-like Modifier Activating Enzyme 1 (UBA1), is a 1058 amino acid (aa) canonical member of the Ubiquitin-activating (E1) enzyme family of proteins with a predicted molecular weight of 118 kDa.  It is ubiquitously expressed and highly conserved; mouse and rat UBE1 share 95% and 96% aa sequence identity with the human UBE1 protein, respectively. UBE1 is found in the cytoplasm and nucleus, and contains a conserved active-site cysteine residue and ATP-binding site common to E1 enzymes (1-3). UBE1 is responsible for the first step in Ubiquitin-protein isopeptide bond formation (4,5). Ubiquitin is activated by UBE1 and thereafter linked to the side chain of a cysteine residue in UBE1, Cys632 in humans, yielding a Ubiquitin-UBE1 conjugate via a thioester bond (5-8). The activated Ubiquitin is then transferred to a lysine residue on the target protein via the Ubiquitin-conjugating  – Ubiquitin ligase enzyme cascade. UBE1 is required for cell cycle progression and has been linked to cellular responses to DNA damage such as nucleotide excision repair (3,9,10). Mutations in UBE1 are associated with X-linked lethal infantile spinal muscular atrophy (11).  UBE1 is a critical component for the initiation of in vitro ubiquitin conjugation reactions.   

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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Ubiquitin-dependent regulation of Cdc42 by XIAP
Authors: A Murali, J Shin, H Yurugi, A Krishnan, M Akutsu, A Carpy, B Macek, K Rajalingam
Cell Death Dis, 2017;8(6):e2900.
Species: Human
Sample Type: Purified Proteins
Application: Bioassay
BAP1 regulates IP3R3-mediated Ca(2+) flux to mitochondria suppressing cell transformation
Authors: A Bononi, C Giorgi, S Patergnani, D Larson, K Verbruggen, M Tanji, L Pellegrini, V Signorato, F Olivetto, S Pastorino, M Nasu, A Napolitano, G Gaudino, P Morris, G Sakamoto, LK Ferris, A Danese, A Raimondi, C Tacchetti, S Kuchay, HI Pass, EB Affar, H Yang, P Pinton, M Carbone
Nature, 2017;546(7659):549-553.
Species: N/A
Sample Type: Protein
Application: Bioassay
DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residues
Authors: V Glorian, J Allègre, J Berthelet, B Dumetier, PM Boutanquoi, N Droin, C Kayaci, J Cartier, S Gemble, G Marcion, D Gonzalez, R Boidot, C Garrido, O Michaud, E Solary, L Dubrez
Cell Death Dis, 2017;8(5):e2816.
Species: Human
Sample Type: Recombinant Protein
Application: Bioassay
DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residues
Authors: V Glorian, J Allègre, J Berthelet, B Dumetier, PM Boutanquoi, N Droin, C Kayaci, J Cartier, S Gemble, G Marcion, D Gonzalez, R Boidot, C Garrido, O Michaud, E Solary, L Dubrez
Cell Death Dis, 2017;8(5):e2816.
Species: Bacteria - E. Coli
Sample Type: Protein
Application: Bioassay
Ubiquitylation of the acetyltransferase MOF in Drosophila melanogaster
Authors: S Schunter, R Villa, V Flynn, JB Heidelberg, AK Classen, P Beli, PB Becker
PLoS ONE, 2017;12(5):e0177408.
Species: Spodoptera frugiperda - Fall Army Worm
Sample Type: Protein
Application: Bioassay
BLADE-ON-PETIOLE proteins act in an E3 ubiquitin ligase complex to regulate PHYTOCHROME INTERACTING FACTOR 4 abundance
Authors: B Zhang, M Holmlund, S Lorrain, M Norberg, L Bakó, C Fankhauser, O Nilsson
Elife, 2017;6(0):.
Species: Human
Sample Type: Recombinant Protein
Application: Bioassay
C-terminus of HSC70-Interacting Protein (CHIP) Inhibits Adipocyte Differentiation via Ubiquitin- and Proteasome-Mediated Degradation of PPAR?
Authors: JH Kim, S Shin, J Seo, EW Lee, M Jeong, MS Lee, HJ Han, J Song
Sci Rep, 2017;7(0):40023.
Species: Human
Sample Type: Cell Lysates
Application: Ubiquitination
Ubiquitination independent of E1 and E2 enzymes by bacterial effectors
Authors: J Qiu, MJ Sheedlo, K Yu, Y Tan, ES Nakayasu, C Das, X Liu, ZQ Luo
Nature, 2016;533(7601):120-4.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
A cell cycle-dependent BRCA1-UHRF1 cascade regulates DNA double-strand break repair pathway choice
Authors: H Zhang, H Liu, Y Chen, X Yang, P Wang, T Liu, M Deng, B Qin, C Correia, S Lee, J Kim, M Sparks, AA Nair, DL Evans, KR Kalari, P Zhang, L Wang, Z You, SH Kaufmann, Z Lou, H Pei
Nat Commun, 2016;7(0):10201.
Species: Human
Sample Type: Purified Proteins
Application: Bioassay
SKP2 loss destabilizes EZH2 by promoting TRAF6-mediated ubiquitination to suppress prostate cancer
Oncogene, 2016;0(0):.
Application: Bioassay
RYBP stimulates PRC1 to shape chromatin-based communication between Polycomb repressive complexes
Elife, 2016;5(0):.
Application: Bioassay
Identification of a novel K311 ubiquitination site critical for androgen receptor transcriptional activity
Nucleic Acids Res., 2016;0(0):.
Application: Bioassay
Regulation of the MDM2-p53 pathway by the nucleolar protein CSIG in response to nucleolar stress
Sci Rep, 2016;6(0):36171.
Species: Human
Sample Type: Protein
Application: Bioassay
The Machado-Joseph Disease Deubiquitinase Ataxin-3 Regulates the Stability and Apoptotic Function of p53
PLoS Biol., 2016;14(11):e2000733.
Application: Bioassay
Identification and Characterization of MCM3 as a Novel KEAP1 Substrate
J Biol Chem, 2016;0(0):.
Species: Human
Sample Type: Protein
Application: Ubiquitination
The San1 ubiquitin ligase functions preferentially with ubiquitin-conjugating enzyme Ubc1 during protein quality control
J Biol Chem, 2016;0(0):.
Application: Bioassay
14-3-3? loss leads to activation of the epithelial to mesenchymal transition due to the stabilization of c-Jun
Authors: Kumarkrishna Raychaudhu
J Biol Chem, 2016;0(0):.
Species: N/A
Sample Type: Protein
Application: Bioassay
A novel cereblon modulator recruits GSPT1 to the CRL4(CRBN) ubiquitin ligase
Authors: Mary E Matyskiela
Nature, 2016;0(0):.
Species: N/A
Sample Type: Protein
Application: Bioassay
hnRNP E1 Protects Chromosomal Integrity by Translational Regulation of Cdc27
Authors: LA Link, BV Howley, GS Hussey, PH Howe
Mol Cancer Res, 2016;0(0):.
Species: Mouse
Sample Type: Cell Lysates
Application: Bioassay
Phosphorylation of CHIP at Ser20 by Cdk5 promotes tAIF-mediated neuronal death.
Authors: Kim C, Yun N, Lee J, Youdim M, Ju C, Kim W, Han P, Oh Y
Cell Death Differ, 2016;23(2):333-46.
Species: N/A
Sample Type: Protein
Application: Enzyme Assay
Deltex1 antagonizes HIF-1alpha and sustains the stability of regulatory T cells in vivo.
Authors: Hsiao H, Hsu T, Liu W, Hsieh W, Chou T, Wu Y, Jiang S, Lai M
Nat Commun, 2015;6(0):6353.
Species: N/A
Sample Type: Protein
Application: Bioassay
Tumour suppressor TRIM33 targets nuclear beta-catenin degradation.
Authors: Xue J, Chen Y, Wu Y, Wang Z, Zhou A, Zhang S, Lin K, Aldape K, Majumder S, Lu Z, Huang S
Nat Commun, 2015;6(0):6156.
Species: Human
Sample Type: Protein
Application: Bioassay
The Rad6/18 ubiquitin complex interacts with the Epstein-Barr virus deubiquitinating enzyme, BPLF1, and contributes to virus infectivity.
Authors: Kumar, Ravindra, Whitehurst, Christop, Pagano, Joseph S
J Virol, 2014;88(11):6411-22.
Species: N/A
Sample Type: Protein
Application: Bioassay
Coordinated regulation of XPA stability by ATR and HERC2 during nucleotide excision repair.
Authors: Lee, T-H, Park, J-M, Leem, S-H, Kang, T-H
Oncogene, 2014;33(1):19-25.
Species: N/A
Sample Type: Protein
Application: Bioassay
Pin1 modulates ERalpha levels in breast cancer through inhibition of phosphorylation-dependent ubiquitination and degradation.
Authors: Rajbhandari P, Schalper K, Solodin N, Ellison-Zelski S, Ping Lu K, Rimm D, Alarid E
Oncogene, 2014;33(11):1438-47.
Species: N/A
Sample Type: Recombinant Protein
Application: Bioassay
Requirement of Smurf-mediated endocytosis of Patched1 in sonic hedgehog signal reception.
Authors: Yue, Shen, Tang, Liu-Ya, Tang, Ying, Tang, Yi, Shen, Qiu-Hong, Ding, Jie, Chen, Yan, Zhang, Zengdi, Yu, Ting-Tin, Zhang, Ying E, Cheng, Steven Y
Elife, 2014;3(0):.
Species: N/A
Sample Type: Protein
Application: Bioassay
Stochastic gate dynamics regulate the catalytic activity of ubiquitination enzymes.
Authors: Rout, Manoj K, Hodge, Curtis D, Markin, Craig J, Xu, Xin, Glover, J N Mark, Xiao, Wei, Spyracopoulos, Leo
J Am Chem Soc, 2014;136(50):17446-58.
Species: N/A
Sample Type: Protein
Application: Bioassay
Suppression of PPARgamma through MKRN1-mediated ubiquitination and degradation prevents adipocyte differentiation.
Authors: Kim J, Park K, Lee E, Jang W, Seo J, Shin S, Hwang K, Song J
Cell Death Differ, 2014;21(4):594-603.
Species: Human
Sample Type: Recombinant Protein
Application: EnzAct
The ubiquitin ligase ASB4 promotes trophoblast differentiation through the degradation of ID2.
Authors: Townley-Tilson, W H Davi, Wu, Yaxu, Ferguson, James E, Patterson, Cam
PLoS ONE, 2014;9(2):e89451.
Species: Human
Sample Type: Whole Cells
Application: Ubiquitination
Dimeric Ube2g2 simultaneously engages donor and acceptor ubiquitins to form Lys48-linked ubiquitin chains.
Authors: Liu, Weixiao, Shang, Yonglian, Zeng, Yan, Liu, Chao, Li, Yanchang, Zhai, Linhui, Wang, Pan, Lou, Jizhong, Xu, Ping, Ye, Yihong, Li, Wei
EMBO J, 2014;33(1):46-61.
Species: N/A
Sample Type: Recombinant Protein
Application: Bioassay
C. elegans ring finger protein RNF-113 is involved in interstrand DNA crosslink repair and interacts with a RAD51C homolog.
Authors: Lee, Hyojin, Alpi, Arno F, Park, Mi So, Rose, Ann, Koo, Hyeon-So
PLoS ONE, 2013;8(3):e60071.
Species: Human
Sample Type: Recombinant Protein
Application: EnzAct
A novel role for inhibitor of apoptosis (IAP) proteins as regulators of endothelial barrier function by mediating RhoA activation.
Authors: Hornburger M, Mayer B, Leonhardt S, Willer E, Zahler S, Beyerle A, Rajalingam K, Vollmar A, Furst R
FASEB J, 2013;28(4):1938-46.
Species: Human
Sample Type: Recombinant Protein
Application: Ubiquitination
A pathogen type III effector with a novel E3 ubiquitin ligase architecture.
Authors: Singer, Alexande, Schulze, Sebastia, Skarina, Tatiana, Xu, Xiaohui, Cui, Hong, Eschen-Lippold, Lennart, Egler, Monique, Srikumar, Tharan, Raught, Brian, Lee, Justin, Scheel, Dierk, Savchenko, Alexei, Bonas, Ulla
PLoS Pathog, 2013;9(1):e1003121.
Species: N/A
Sample Type: Recombinant Protein
Application: Bioassay
Structural determinants of ubiquitin conjugation in Entamoeba histolytica.
Authors: Bosch D, Siderovski D
J Biol Chem, 2013;288(4):2290-302.
Species: Amoeba - Entamoeba histolytica
Sample Type: Recombinant Protein
Application: Bioassay
Ubiquitination increases parkin activity to promote autophagic alpha-synuclein clearance.
Authors: Lonskaya I, Desforges N, Hebron M, Moussa C
PLoS ONE, 2013;8(12):e83914.
Species: N/A
Sample Type: Recombinant Protein
Application: Enzyme Assay
Characterization of ML-IAP protein stability and physiological role in vivo.
Authors: Varfolomeev, Eugene, Moradi, Elham, Dynek, Jasmin N, Zha, Jiping, Fedorova, Anna V, Deshayes, Kurt, Fairbrother, Wayne J, Newton, Kim, Le Couter, Jennifer, Vucic, Domagoj
Biochem J, 2012;447(3):427-36.
Species: Human
Sample Type: Protein
Application: Bioassay
A subset of mixed lineage leukemia proteins has plant homeodomain (PHD)-mediated E3 ligase activity.
Authors: Wang, Jingya, Muntean, Andrew G, Wu, Laura, Hess, Jay L
J Biol Chem, 2012;287(52):43410-6.
Species: Human
Sample Type: Recombinant Protein
Application: Ubiquination
TRIM16 acts as an E3 ubiquitin ligase and can heterodimerize with other TRIM family members.
Authors: Bell JL, Malyukova A, Holien JK
PLoS ONE, 2012;7(5):e37470.
Species: Human
Sample Type: Cell Lysates
Application: Bioassay
DNA damage-induced heterogeneous nuclear ribonucleoprotein K sumoylation regulates p53 transcriptional activation.
Authors: Pelisch F, Pozzi B, Risso G, Munoz M, Srebrow A
J Biol Chem, 2012;287(36):30789-99.
Species: N/A
Sample Type: Protein
Application: Bioassay
Inhibition of a NEDD8 Cascade Restores Restriction of HIV by APOBEC3G.
Authors: Stanley D, Bartholomeeusen K, Crosby D, Kim D, Kwon E, Yen L, Cartozo N, Li M, Jager S, Mason-Herr J, Hayashi F, Yokoyama S, Krogan N, Harris R, Peterlin B, Gross J
PLoS Pathog, 2012;8(12):e1003085.
Species: N/A
Sample Type: Recombinant Protein
Application: Bioassay

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