抗alpha Tubulin [GT114]抗体 | Anti-alpha Tubulin [GT114] antibody

掲載日情報:2020/04/02 現在Webページ番号:194323

GeneTex社の抗alpha Tubulin [GT114]抗体 | Anti-alpha Tubulin [GT114] antibodyです。

本製品は研究用です。研究用以外には使用できません。

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GeneTex社では製品を使用したユーザーからのレビューを各国から集め、GeneTex社ホームページで公開しています。各ユーザーの使用アプリケーション、試料の由来動物、希釈倍率、ブロッキング試薬といった実験条件, 実験結果画像など参考となる情報が掲載されています。
抗alpha Tubulin [GT114]抗体(#GTX628802)のユーザーレビューはこちら。

Ratingrating star full rating star full rating star full rating star full rating star empty( Average 4 based on 2 users reviews)
Application
Western Blot(WB)rating star full rating star full rating star full rating star full rating star full( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)rating star full rating star full rating star full rating star empty rating star empty( Average 3 based on 1 users reviews)

特長

  • 高品質の抗体です。
  • 幅広い研究分野に関連する抗体を取り揃えています。
  • 使用されたアプリケーションや動物種などの情報が充実しています。
  • 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

[在庫・価格 :2024年03月28日 20時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-alpha Tubulin, Mouse-Mono(GT114)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 113
説明文
レビューあり
別名:K-ALPHA-1 antibody,TUBA1B antibody,alpha-tubulin ubiquitous antibody,alpha tubulin antibody,tubulin K-alpha-1 antibody,tubulin alpha-ubiquitous chain antibody,tubulin,alpha,ubiquitous antibody,tubulin alpha-1B chain antibody,tubulin,alpha 1b antibody
クローン:GT114
Genbank No: 10376
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Mouse クラス IgG 標識 Unlabeled
交差性 Crucian carp/Drosophila/Goat/Human/Mouse/Rat/Zebrafish 適用 FCM,IC,IF,IHC,Western Blot
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事 ローディング・コントロール用抗体
GeneTex社 オルガネラマーカー抗体
ゼブラフィッシュ(zebrafish)研究用製品特集
使いっきり抗体
関連記事 GeneTex社における抗体の品質管理
Anti-alpha Tubulin, Mouse(GT114)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 113
説明文
レビューあり
別名:K-ALPHA-1 antibody,TUBA1B antibody,alpha-tubulin ubiquitous antibody,alpha tubulin antibody,tubulin K-alpha-1 antibody,tubulin alpha-ubiquitous chain antibody,tubulin,alpha,ubiquitous antibody,tubulin alpha-1B chain antibody,tubulin,alpha 1b antibody
クローン:GT114
Genbank No: 10376
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Mouse クラス IgG 標識 Unlabeled
交差性 Crucian carp/Drosophila/Goat/Human/Mouse/Rat/Zebrafish 適用 FCM,IC,IF,IHC,Western Blot
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事 GeneTex社 オルガネラマーカー抗体
ローディング・コントロール用抗体
ゼブラフィッシュ(zebrafish)研究用製品特集
関連記事 GeneTex社における抗体の品質管理

[在庫・価格 :2024年03月28日 20時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-alpha Tubulin, Mouse-Mono(GT114)

文献数: 113

説明文 レビューあり
別名:K-ALPHA-1 antibody,TUBA1B antibody,alpha-tubulin ubiquitous antibody,alpha tubulin antibody,tubulin K-alpha-1 antibody,tubulin alpha-ubiquitous chain antibody,tubulin,alpha,ubiquitous antibody,tubulin alpha-1B chain antibody,tubulin,alpha 1b antibody
クローン:GT114
Genbank No: 10376
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Mouse
交差性 Crucian carp/Drosophila/Goat/Human/Mouse/Rat/Zebrafish 適用 FCM,IC,IF,IHC,Western Blot
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事 ローディング・コントロール用抗体
GeneTex社 オルガネラマーカー抗体
ゼブラフィッシュ(zebrafish)研究用製品特集
使いっきり抗体
関連記事 GeneTex社における抗体の品質管理

Anti-alpha Tubulin, Mouse(GT114)

文献数: 113

説明文 レビューあり
別名:K-ALPHA-1 antibody,TUBA1B antibody,alpha-tubulin ubiquitous antibody,alpha tubulin antibody,tubulin K-alpha-1 antibody,tubulin alpha-ubiquitous chain antibody,tubulin,alpha,ubiquitous antibody,tubulin alpha-1B chain antibody,tubulin,alpha 1b antibody
クローン:GT114
Genbank No: 10376
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Mouse
交差性 Crucian carp/Drosophila/Goat/Human/Mouse/Rat/Zebrafish 適用 FCM,IC,IF,IHC,Western Blot
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事 GeneTex社 オルガネラマーカー抗体
ローディング・コントロール用抗体
ゼブラフィッシュ(zebrafish)研究用製品特集
関連記事 GeneTex社における抗体の品質管理



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DATA IMAGES

alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX628802) diluted at a dilution of 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
alpha Tubulin antibody [GT114] detects alpha Tubulin protein expression by immunofluorescent analysis.Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha Tubulin protein stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:250.Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Immunofluorescence photomicrographs of frozen sections of mouse brain.Green: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:200. The signal was developed using goat anti-rmouse IgG antibody (Dylight488) (GTX213111-04).Blue: Nuclear staining with Hoechst 33342.
alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in human cervix by immunohistochemical analysis. Sample: Paraffin-embedded human cervix. alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon.Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500.Blue: Hoechst 33342 staining. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis. Varied amount of 293T Whole cell extracts was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX628802) at a dilution of 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha Tubulin antibody [GT114] detects TUBA1B protein by western blot analysis.A. 30 μg zebrafish eye lysate/extract10% SDS-PAGEalpha Tubulin antibody [GT114] (GTX628802) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
TUBA1B antibody detects TUBA1B protein at Cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in -20ºC 100% methanol for 5 min.Green: TUBA1B protein stained by TUBA1B antibody (GTX628802) diluted at 1:500.Blue: Hoechst 33342 staining.
alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha Tubulin stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 μm.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
alpha Tubulin antibody [GT114] detects TUBA1B protein by western blot analysis.A. 30 μg 293T whole cell lysate/extract B. 30 μg NIH-3T3 whole cell lysate/extract C. 30 μg mouse brain lysate/extract D. 30 μg PC-12 whole cell lysate/extract E. 30 μg rat brain lysate/extract F. 30 μg drosophila lysae/extract10% SDS-PAGEalpha Tubulin antibody [GT114] (GTX628802) dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Various whole cell extracts (15 | 5 | 2 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.


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製品情報

HostMouse
ClonalityMonoclonal
Clone NameGT114
IsotypeIgG2a
ApplicationWB, ICC/IF, IHC-P, IHC-Fr
ReactivityHuman, Mouse, Rat, Zebrafish, Drosophila


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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW 50 kDa. ( Note )
Positive Control 293T , A431 , HeLa , HepG2 , NIH-3T3 , mouse brain , PC-12 , rat brain , drosophila , zebrafish eye , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , *U87-MG
Predict Reactivity Rabbit, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)


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PROPERTIES

Form Liquid
Buffer PBS
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species Human
Immunogen Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.
Purification Affinity purified by Protein A.
From tissue culture supernatant
Conjugation Unconjugated
Note For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


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TARGET

Synonyms K-ALPHA-1 antibody, TUBA1B antibody, alpha-tubulin ubiquitous antibody, alpha tubulin antibody, tubulin K-alpha-1 antibody, tubulin alpha-ubiquitous chain antibody, tubulin, alpha, ubiquitous antibody, tubulin alpha-1B chain antibody, tubulin, alpha 1b antibody
Background Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.


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REFERENCEE(抜粋)

Application ReferenceApplication/Reactivity
Yang H et al. Nat Commun 2020; 11 (1) : 632 Understanding the structural basis of HIV-1 restriction by the full length double-domain APOBEC3G.Application : WB
Reactivity : Human
Huang SW et al. J Cell Mol Med 2020; 24 (2) : 1822-1836 Lovastatin-mediated MCF-7 cancer cell death involves LKB1-AMPK-p38MAPK-p53-survivin signalling cascade.Application : WB
Reactivity : Human
Lieschke S et al. Cells 2019; 9 (1) CCL11 Differentially Affects Post-Stroke Brain Injury and Neuroregeneration in Mice Depending on Age.Application : WB
Reactivity : Mouse
Zhang J et al. BMC Cancer 2019; 19 (1) : 921 Lentivirus-mediated CDglyTK gene-modified free flaps by intra-artery perfusion show targeted therapeutic efficacy in rat model of breast cancer.Application : WB
Reactivity : Human
Teng LL et al. PLoS Biol 2019; 17 (3) : e2007097 Serotonin receptor HTR6-mediated mTORC1 signaling regulates dietary restriction-induced memory enhancement.


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お問い合わせ先

(テクニカルサポート 試薬担当)

reagent@funakoshi.co.jp

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