Human VEGF Quantikine ELISA Kit | hVEGF QKit
掲載日情報：2017/09/01 現在Webページ番号：111227
R&D Systems製のHuman VEGFを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月25日 19時55分現在]
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VEGF, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2024年04月25日 19時55分現在]
VEGF, Human, ELISA Kit, Quantikine (96well)
文献数： 879
- 商品コード：DVE00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物,カルタヘナ該当品
| 説明文 |
測定範囲：15.60 - 1000 pg/mL (Cell Culture Supernates), 31.30 - 2000 pg/mL (Serum, Heparin Plasma, Citrate Plasma, EDTA Plasma)，感度：5 pg/mL (Cell Culture Supernates), 9 pg/mL (Serum, Heparin Plasma, Citrate Plasma, EDTA Plasma)，測定試料：Cell Culture Supernates (200 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC01-1，測定時間：4.5 hours，測定因子：VEGF 別名：MVCD1 Genbank No： 7422 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | 医薬用外毒物,カルタヘナ該当品 | ||||||
| 保存条件 | 4℃ | 法規備考 | |||||
| 掲載カタログ |
ニュース2021年9月15日号 p.18
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| 製品記事 |
Quantikine Human VEGF ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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- ELISA kitトラブルシューティングガイド／FAQ
- Product Details
- Product Summary
- Precision
- Recovery
- Linearity
- Additional Infomation
- Citations
ELISA kitトラブルシューティングガイド／FAQ
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (200 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL)
- Sensitivity
- 9 pg/mL
- Assay Range
- 15.6 - 1,000 pg/mL (Cell Culture Supernates), 31.2 - 2,000 pg/mL (Serum, Heparin Plasma, Citrate Plasma, EDTA Plasma)
- Specificity
- Natural and recombinant human VEGF
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine Human VEGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure VEGF165 levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human VEGF165 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human VEGF and recombinant human VEGF121 showed linear curves that were parallel to the standard curves obtained using the Quantikine Human VEGF Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human VEGF.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of a known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 53.7 | 235 | 910 | 64.5 | 250 | 1,003 |
| Standard Deviation | 3.6 | 10.6 | 46.2 | 5.7 | 17.4 | 61.7 |
| CV% | 6.7 | 4.5 | 5.1 | 8.8 | 7 | 6.2 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 29.1 | 123 | 531 | 32.8 | 128 | 495 |
| Standard Deviation | 1.9 | 5 | 18.4 | 2.8 | 6.4 | 33 |
| CV% | 6.5 | 4.1 | 3.5 | 8.5 | 5 | 6.7 |
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Recovery
The recovery of human VEGF spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=5) | 102 | 92-115 |
| Heparin Plasma (n=5) | 93 | 82-102 |
| Citrate Plasma (n=5) | 100 | 88-113 |
| EDTA Plasma (n=5) | 97 | 82-113 |
| Cell Culture Media (n=5) | 102 | 95-111 |
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Linearity
To assess the linearity of the assay, samples spiked with high concentrations of VEGF were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. 
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Additional Infomation
- Molecule Information
- VEGF
- Aliases
- VAS; Vasculotropin; VEGFA; VPF
- Entrez Gene IDs
- 7422 (Human); 22339 (Mouse); 83785 (Rat); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
- Background
- VEGF
- Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) or vasculotropin, is a homodimeric 34 - 42 kDa, heparin-binding glycoprotein with potent angiogenic, mitogenic and vascular permeability-enhancing activities specific for endothelial cells. The amino acid sequence of VEGF exhibits primary structural, as well as limited amino acid sequence, homology with that of the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfide bonds are conserved among these growth factors. A cDNA encoding a protein having a 53% amino acid sequence homology in the PDGF-like region of VEGF has been isolated from a human placental cDNA library. This protein, named placenta growth factor (PlGF), is now recognized to be a member of the VEGF family of growth factors. Based on its homology with VEGF, PlGF was also proposed to be an angiogenic factor. Two receptor tyrosine kinases have been described as putative VEGF receptors. Flt-1 (fms-like tyrosine kinase), and KDR (kinase-insert-domain-containing receptor) proteins have been shown to bind VEGF with high affinity.In vitro, VEGF is a potent endothelial cell mitogen. In cultured endothelial cells, VEGF can activate phospholipase C and induce rapid increases of free cytosolic Ca2+. VEGF has been shown to stimulate von Willebrand factor release from endothelial cells and induce expression of tissue factor activity in endothelial cells as well as in monocytes. VEGF has also been shown to be chemotactic for monocytes and osteoblasts. In vivo, VEGF can induce angiogenesis as well as increase microvascular permeability. As a vascular permeability factor, VEGF acts directly on the endothelium and does not degranulate mast cells. It promotes extravasation of plasma fibrinogen, leading to fibrin deposition which alters the tumor extracellular matrix. The modified extracellular matrix subsequently promotes the migration of macrophages, fibroblasts and endothelial cells. Based on its in vitro and in vivo properties, VEGF is expected to play important roles in inflammation and during normal and pathological angiogenesis, a process that is associated with wound healing, embryonic development, and growth and metastasis of solid tumors. Elevated levels of VEGF have been reported in synovial fluids of rheumatoid arthritis patients and in sera from cancer patients.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Transcriptional repression of VEGF by ZNF24: mechanistic studies and vascular consequences in vivo.
Chan , Joanne, D'Amore , Patricia, Filingeri , Domenic, Hasso , Sean M, Jia , Di, Moses , Marsha A, Pampo , Christin, Rice , Lori, Rodig , Scott J, Siemann , Dietmar , Zurakowski , David
Blood 2013 121:707-15
Species: Human
Sample Type: Cell Culture Supernates
Caspase 3 involves in neuroplasticity, microglial activation and neurogenesis in the mice hippocampus after intracerebral injection of kainic acid.
Chang , Luping, Hsu , Chia-Lin, Huang , Fong-Lee, Lai , Tzu-Hsua, Shiao , Young-Ji, Tsay , Huey-Jen, Tzeng , Tsai-Ten
J Biomed Sci 2013 20:90
Species: Human
Sample Type: Cell Culture Supernates
The role of SDF-1-CXCR4/CXCR7 axis in biological behaviors of adipose tissue-derived mesenchymal stem cells in vitro.
Jin P, Li Q, Li X, Tao C, Zhang A
Biochem Biophys Res Commun 2013 441:675-80
Species: Human
Sample Type: Cell Culture Supernates
Hypoxia potentiates glioma-mediated immunosuppression.
Fokt I, Fuller G, Heimberger AB, Kong LY, Melillo G, Priebe W, Wang Y, Wei J, Wu A
PLoS ONE 2012 6:e16195
Species: Human
Sample Type: Cell Culture Supernates
Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma.
Caillat-Zucman S, Choukem SP, Dupuy S, Lambert M, Lebbe C, Pages C, Tognarelli S, Zucman D
PLoS Pathog. 2012 8:e1002486
Species: Human
Sample Type: Cell Culture Supernates
Bevacizumab attenuates major signaling cascades and eIF4E translation initiation factor in multiple myeloma cells.
Attar-Schneider O, Drucker L, Zismanov V
Lab. Invest. 2012 92:178-90
Species: Human
Sample Type: Cell Culture Supernates
Late SV40 factor (LSF) enhances angiogenesis by transcriptionally up-regulating matrix metalloproteinase-9 (MMP-9).
Chen D, Gredler R, Santhekadur PK
J. Biol. Chem. 2012 287:3425-32
Species: Human
Sample Type: Cell Culture Supernates
Plasma biomarkers as predictors of outcome in patients with advanced hepatocellular carcinoma.
Amadori D, Arvanitakis C, Badulescu F, Barrios C, Beaugrand M, Befeler A, Bennouna J, Blanc J-, Blum H, Bolondi L, Borbath I, Boyer M, Boyer T, Britten C, Bronowicki J-, Bruix J, Buggisch P, Burak K, Bustamante Schneider J, Byrne T, Caspary W, Cisnero Garza LE, Cognetti F, Cosme de Oliveira A, Craxi A, De Greve J, Degos F, Diago M, Dollinger M, Dominguez S, Falk S, Farinati F, Figer A, Forner A, Gamargo Garate C, Gane E, Garcia-Tsao G, Gerken G, Germanidis G, Goke B, Gold P, Goldenberg A, Grange J-, Gregor M, Greten T, Gridelli C, Haussinger D, Heuman D, Hilgard P, Hillon P, Jassem J, Jeffry Evans TR, Katsos I, Kennedy P, Koch A, Kotzev I, Kubyshkin V, Lathia CD, Leon J, Llovet JM, Lozano A, Ma M, Marrero J, Martinez-Castillo J, Martoni A, Mazzaferro V, Meinhardt G, Metrakos P, Meyer T, Miron L, Montero Alvarez JL, Muellhaupt B, O'Donnell A, Olweny C, Pallota MG, Pascual S, Pena CE, Porta C, Raoul J-, Reeves H, Ricci S, Riordan S, Ross P, Roth A, Ru??z del Arbol L, Rydzewska G, Sangiovanni A, Sangro B, Santoro A, Scher??bl J, Scheulen M, Schilsky M, Schmid R, Schwartz J, Schwartz M, Seitz J-, Shan M, Sherman M, Sol?? R, Spengler U, Stemmer S, Strickland A, Szawlowski A, Tabernero J, Takov D, Tchernev K, Tebbutt N, Thomson B, Tomczak P, Trevisani F, Van Laethem J-, Van Steenbergen W, Van Vlierberghe H, Wiest R, Zarba JJ, Zeuzem S
Clin. Cancer Res. 2012 18:2290-300
Species: Human
Sample Type: Plasma
Cardiac angiogenic imbalance leads to peripartum cardiomyopathy.
Arany Z, Bauersachs J, Burke SD, del Monte F, Farrell C, Hacker MR, Hess P, Hilfiker-Kleiner D, Jang C, Karumanchi SA, Khankin EV, Koulisis N, Liu L, Mahmood F, Mitchell J, Patten IS, Rana S, Rhee JS, Rowe GC, Shahul S, Tudorache I
Nature 2012 485:333-8
Species: Human
Sample Type: Plasma
Semaphorin 4A exerts a proangiogenic effect by enhancing vascular endothelial growth factor-A expression in macrophages.
De Pizzol M, Meda C, Molla F
J. Immunol. 2012 188:4081-92
Species: Human
Sample Type: Cell Culture Supernates
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