抗TRA-1-60抗体(Anti-TRA-1-60, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	2102Ep human embryonal carcinoma cell line
Source	Monoclonal Mouse IgM Clone # TRA-1-60
Purification	IgM-specific Affinity-purified from hybridoma culture supernatant
Specificity	Detects human TRA‑1‑60(R).

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
CyTOF-reported	This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Flow Cytometry
	Detection of TRA‑1‑60 in BG01V Human Cells by Flow Cytometry BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA‑1‑60 Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770, filled histogram) or isotype control antibody followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116).
Immunocytochemistry
	TRA‑1‑60 in BG01V Human Stem Cells. TRA‑1‑60 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA‑1‑60 Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (yellow; Catalog # NL019) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	TRA‑1‑60(R) in ADLF1 and FAB2 Stem Cell Lines. TRA‑1‑60(R) was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Mouse Anti-Human TRA‑1‑60(R) Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

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Related Product & Information

Background

TRA-1-60(R)

background_content

Background: TRA-1-60(R) TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells (1). The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors (2‑4). Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan (5). Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope (6).

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Citations

1. Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience
   Authors: PA De Sousa, R Steeg, E Wachter, K Bruce, J King, M Hoeve, S Khadun, G McConnachi, J Holder, A Kurtz, S Seltmann, J Dewender, S Reimann, G Stacey, O O'Shea, C Chapman, L Healy, H Zimmermann, B Bolton, T Rawat, I Atkin, A Veiga, B Kuebler, BM Serano, T Saric, J Hescheler, O Brüstle, M Peitz, C Thiele, N Geijsen, B Holst, C Clausen, M Lako, L Armstrong, SK Gupta, AJ Kvist, R Hicks, A Jonebring, G Brolén, A Ebneth, A Cabrera-So, P Foerch, M Geraerts, TC Stummann, S Harmon, C George, I Streeter, L Clarke, H Parkinson, PW Harrison, A Faulconbri, L Cherubin, T Burdett, C Trigueros, MJ Patel, C Lucas, B Hardy, R Predan, J Dokler, M Brajnik, O Keminer, O Pless, P Gribbon, C Claussen, A Ringwald, B Kreisel, A Courtney, TE Allsopp
   Stem Cell Res, 2017;20(0):105-114.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.
   BMC Vet Res, 2012;8(0):150.
   Species: Canine
   Sample Type: Whole Cells
   Application: Flow


3. Comparative proteomic analysis of human somatic cells, induced pluripotent stem cells, and embryonic stem cells.
   Authors: Kim SY, Kim MJ, Jung H
   Stem Cells Dev., 2012;21(8):1272-86.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC

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