Human Endothelin-1 QuantiGlo ELISA Kit
掲載日情報：2016/08/18 現在Webページ番号：111381
R&D Systems製のHuman Endothelin-1を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年06月03日 00時00分現在]
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Endothelin-1, Human, ELISA Kit, QuantiGlo, 2nd Generation (96 well) |
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[在庫・価格 ：2024年06月03日 00時00分現在]
Endothelin-1, Human, ELISA Kit, QuantiGlo, 2nd Generation (96 well)
文献数： 46
- 商品コード：QET00B
- メーカー：RSD
- 包装：1kit
- 価格：¥135,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：0.34 - 250 pg/mL，感度：0.102 pg/mL，測定試料：Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Urine (100 uL)，種交差性：Human，検出方法：化学発光，アッセイ法：サンドイッチELISA，アッセイ数：96 well，別売品コントロール：#QC197，測定時間：4.5 hours，測定因子：Endothelin-1 別名：EDN1 Genbank No： 1906 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
QuantiGlo Chemiluminescent Sandwich ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Urine (100 µL)
- Sensitivity
- 0.102 pg/mL
- Assay Range
- 0.34 - 250 pg/mL (Serum, Cell Culture Supernates, EDTA Plasma, Urine)
- Specificity
- Natural and synthetic human Endothelin-1
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The QuantiGlo Human Endothelin-1 Chemiluminescent Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Endothelin-1 levels in cell culture supernates, serum, EDTA plasma, and urine without extraction. It contains synthetic human Endothelin-1 and antibodies raised against the synthetic factor. This immunoassay has been shown to accurately quantitate human Endothelin-1. Results obtained using natural human Endothelin-1 showed dose curves that were parallel to the standard curves obtained using the QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values of natural human Endothelin-1.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 1.78 | 20.5 | 90.9 | 1.76 | 19.8 | 86.8 |
| Standard Deviation | 0.06 | 0.7 | 2.4 | 0.16 | 1.3 | 3.9 |
| CV% | 3.4 | 3.4 | 2.6 | 9.1 | 6.6 | 4.5 |
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Recovery
The recovery of synthetic human Endothelin-1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 101 | 93-107 |
| Urine (n=4) | 98 | 92-105 |
| Serum (n=4) | 95 | 87-103 |
| EDTA Plasma (n=4) | 94 | 88-99 |
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Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of Endothelin-1 in various matrices were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Endothelin-1
- Aliases
- EDN1; ET1
- Entrez Gene IDs
- 1906 (Human); 13614 (Mouse); 24323 (Rat)
- Background
- Endothelin-1
- Endothelin-1 (ET-1) is produced by many tissues including lung, kidney, brain, endocrine glands, and placenta. It is also expressed by several cell types including, mast cells, endothelial cells, epithelial and smooth muscle cells. The most prominent source is vascular endothelium. ET-1 is best known as a potent vasoconstrictor. Elevated blood ET-1 levels are associated with a variety of diseases, possibly as a function of a stress response.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Daily red wine consumption improves vascular function by a soluble guanylyl cyclase-dependent pathway.
Botden IP, Langendonk JG, Meima ME
Am. J. Hypertens. 2011 24:162-8
Species: Human
Sample Type: Plasma
Gingival fibroblasts display reduced adhesion and spreading on extracellular matrix: a possible basis for scarless tissue repair?
Carter DE, Guo F, Leask A, Mukhopadhyay A
PLoS ONE 2011 6:e27097
Species: Human
Sample Type: Cell Culture Supernates
Endothelin receptors blockade blunts hypoxia-induced increase in PAP in humans.
Azizi M, Peyrard S, Pham I, Richalet JP, Wuerzner G
Eur. J. Clin. Invest. 2010 40:195-202
Species: Human
Sample Type: Plasma
Early life stress downregulates endothelin receptor expression and enhances acute stress-mediated blood pressure responses in adult rats.
D'Angelo G, Loria AS, Pollock DM, Pollock JS
Am. J. Physiol. Regul. Integr. Comp. Physiol. 2010 299:R185-91
Species: Rat
Sample Type: Plasma
Vehicular emissions induce vascular MMP-9 expression and activity associated with endothelin-1-mediated pathways.
Campen MJ, Knuckles TL, Lucas S, Lucero J, Lund AK, Madden MC, McDonald JD, Seagrave JC
Arterioscler. Thromb. Vasc. Biol. 2009 29:511-7
Species: Human
Sample Type: Plasma
Effects of nonoral estradiol-micronized progesterone or low-dose oral estradiol-drospirenone therapy on metabolic variables and markers of endothelial function in early postmenopause.
Casanova G, Lhullier F, Radavelli S, Spritzer PM
Fertil. Steril. 2009 92:605-12
Species: Human
Sample Type: Plasma
Endothelial function, endothelin-1, and fibrinogen in young women using the vaginal contraceptive ring.
Kaplan PF, Meendering JR, Miller NP, Minson CT, Torgrimson BN
Fertil. Steril. 2009 92:441-7
Species: Human
Sample Type: Serum
Regulation of angiogenic factors by HDM2 in renal cell carcinoma.
Ashcroft M, Carroll VA
Cancer Res. 2008 68:545-52
Species: Human
Sample Type: Cell Culture Supernates
Eosinophil-derived cationic proteins activate the synthesis of remodeling factors by airway epithelial cells.
Gleich GJ, Pegorier S, Pretolani M, Wagner LA
J. Immunol. 2006 177:4861-9
Species: Human
Sample Type: Cell Culture Supernates
Digital blood flow and plasma endothelin concentration in clinically endotoxemic horses.
Bailey SR, Elliott J, Katz L, Marr CM, Menzies-Gow NJ, Stevens K
Am. J. Vet. Res. 2005 66:630-6
Species: Equine
Sample Type: Plasma
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